A simple colorimetric assay for aspartate aminotransferase.
نویسندگان
چکیده
منابع مشابه
Aspartate Aminotransferase and Aianine Aminotransferase Doubie Kinetic Assay in a Single Channel of the Hitachi 705
mine by this method the intra-erythrocytic concentrations of this drug after preliminary extraction. After centrifugation, serum is separated and erythrocytes are washed two times with isotonic saline, then erythrocytes are hemolyzed at -18 #{176}C. For the extraction, mix 1 roL of erythrocytes with five drops of glacial acetic acid and 5 mL of isopropanol in chloroform (5/95 by vol), vortex-mi...
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Aspartate aminotransfcrase (L-aspartate: 2-oxoglutarate aminotransferase, EC 2. 6.1.1) comprises two isoen&ymcs. One is anionic, is associated with the soluble fraction of the cell and ordinarily forms the bulk of the scrum activity. The other is cationic, and is associated with the mitochondria (1—7). Each may in turn be subdivisible (8—10), but it is unlikely that this will influence the pres...
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The isoenzymes of aspartate transaminase differ in their kinetic properties in that the cytoplasmic isoenzyme is more readily inhibited by adipate and by 2-oxoglutarate (substrate) at low pH. A differential kinetic assay based on this phenomenon has been optimised for use in assays of serum samples. The new method agrees well with an immune absorption procedure. Methods based on chromatographic...
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The cofactor pyridoxal 5’-phosphate bound through an aldimine linkage to lysine residues of the enzyme aspartate amiuotransferase is very stable to irradiation with light of 420 nm. The catalytic function of the enzyme exposed to light absorbed by the cofactor remains unaffected, indicating that pyridoxal 5’-phosphate is not an efficient active site photosensitizer for this aminotransferase. Th...
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1. Acetylation of aspartate aminotransferase from pig heart inhibits completely the enzymic activity when the coenzyme is in the amino form (pyridoxamine phosphate) or when the coenzyme has been removed, but not when the coenzyme is in the aldehyde form (pyridoxal phosphate). 2. The group the acylation of which is responsible for the inhibition has been identified with the in-amino group of a l...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1984
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.48.2819